KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] | Abcam | Abcam Cat# ab110330, RRID:AB_10858459 |
| Recombinant Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody [EPR12200] | Abcam | Abcam Cat# ab177461, RRID:AB_2756339 |
| Anti-MCU antibody | Abcam | Cat#ab121499 |
| Tom20 (D8T4N) Rabbit mAb | Cell Signaling Technology | Cell Signaling Technology Cat# 42406, RRID:AB_2687663 |
| Pyruvate Dehydrogenase (C54G1) Rabbit mAb | Cell Signaling Technology | Cell Signaling Technology Cat# 3205, RRID:AB_2162926 |
| Bacterial and Virus Strains | ||
| pCMV-CEPIA3mt | Suzuki et al. | Addgene Cat#58219, RRID: addgene_58219 |
| Lenti-X Packaging Single Shots (VSV-G) | Takara | Cat#631275 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) | Sigma | Cat# C2920-10MG |
| JC-1 Dye (Mitochondrial Membrane Potential Probe) | ThermoFisher Scientific | Cat#T3168 |
| Calcium Green-5N, Hexapotassium Salt, cell impermeant | ThermoFisher Scientific | Cat#C3737 |
| Fura-2, AM, cell permeant | ThermoFisher Scientific | Cat#F1221 |
| Probenecid | Enzo | Cat# ALX-430-113-G005 |
| Pluronic F-127 (20% solution in DMSO) | ThermoFisher Scientific | Cat#P3000MP |
| Ionomycin | Cayman Chemical | Cat#10004974 |
| MitoTracker Red | ThermoFisher Scientific | Cat#M22425 |
| CypHer5E NHS Ester | GE healthcare | Cat#PA15401 |
| BAM15 | Cayman Chemical | Cat#17811 |
| Antimycin A | Sigma | Cat# A8674-25MG |
| Rotenone | Sigma | Cat#R8875-1G |
| Zymosan A S. cerevisiae BioParticles, Texas Red conjugate | ThermoFisher Scientific | Cat#Z2843 |
| Zymosan A S. cerevisiae BioParticles, unlabeled | ThermoFisher Scientific | Cat#Z2849 |
| Dorsomorphin dihydrochloride | Tocris | Cat#3093 |
| 6-Aminonicotinamide | Cayman Chemical | Cat#10009315 |
| 6,8-Bid(benzylthio)octanoic acid | Tocris | Cat#5348 |
| BAPTA-AM, cell permeant chelator | ThermoFisher Scientific | Cat#B6769 |
| Beta-glucan peptide | Invivogen | Cat#tlrl-bgp |
| Thapsigargin | ThermoFisher Scientific | Cat#T7458 |
| Critical Commercial Assays | ||
| CellTiter-Glo® 2.0 Cell Viability Assay | Promega | Cat#G9241 |
| CellTrace Violet Cell Proliferation Kit, for flow cytometry | ThermoFisher Scientific | Cat#C34557 |
| CellTrace Yellow Cell Proliferation Kit, for flow cytometry | ThermoFisher Scientific | Cat#C34567 |
| CellROX Green Reagent, for oxidative stress detection | ThermoFisher Scientific | Cat#C10444 |
| Pierce LDH Cytotoxicity Assay Kit | ThermoFisher Scientific | Cat#88953 |
| Experimental Models: Cell Lines | ||
| RAW264.7 ATCC TIB-71 | ATCC | ATCC Cat# TIB-71, RRID:CVCL_0493 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae sy1022 fy5 | This paper | Gift: Jeff Smith (UVA) |
| Culti-Loops Candida albicans ATCC 10231 | ThermoFisher Scientific | Cat#R4601503 |
| Mouse: B6;129S-Mcutm1.1Jmol/J | The Jackson Laboratory | IMSR Cat# JAX:029817, RRID:IMSR_JAX:029817 |
| Mouse: B6J.B6N(Cg)-Cx3cr1tm1.1(cre)/Jung/J | The Jackson Laboratory | IMSR Cat# JAX:025524, RRID:IMSR_JAX:025524 |
| Oligonucleotides | ||
| MCU Exon 5 Forward Primer | This Paper | N/A |
| 5’-TGAACGACGTGAAGACCCT G-3’ | ||
| MCU Exon 5 Reverse Primer | This Paper | N/A |
| 5’-TTCGTACCTTCTCCAGGGGG-3’ | ||
| Software and Algorithms | ||
| ImageJ | https://imagej.nih.gov/ij/ | |
| SPCM | Wallrabe et al., 2018 | https://www.becker-hickl.com/products/spcm/ |
| Graphpad Prism | GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ |
| Seahorse Wave | Agilent | https://www.agilent.com/en/products/cell-analysis/software-download-for-wave-desktop |
| Other | ||
| CFX Connect Real-Time system (Bio-Rad) | qPCR | N/A |
| XF24 Extracellular Flux Analyzer (Agilent Technologies) | Seahorse | N/A |
| FlexStation 3 | In vitro mitochondrial Ca2+ uptake, LDH assay, ATP assay | N/A |
| Leica SP5 × Confocal/Spectral Imaging Microscopy System with White Light Laser | Live mCa2+ imaging for RAW-3mt | N/A |
| Zeiss Axio Observer with DG4 Illuminator and ORCA-Flash 4.0 V2 CMOS camera | Fura-2 Ca2+ imaging | N/A |
| LSM880 confocal and a Chameleon multiphoton light source | Immunofluorescence | N/A |
| Attune NxT equipped with four lasers (488nm, 640nm, 405nm, and 561nm) | Flow Cytometry | N/A |
| JEOL 1230 Transmission Electron Microscope | TEM | N/A |
| FUIJ Film LAS-4000 | Western Blotting | N/A |