Fig 2. Activity of RodA and PBP2 are not required for their interaction.

a, Phase contrast images of the complementation of RodA variants. RodA temperature sensitive strain LMC882 was transformed with plasmids expressing RodA variants and grown in LB medium at 30°C (left panel) and 42°C (right panel) for 2 mass doublings (with15 μM IPTG induction). EV, empty vector. b, Calculated acceptor FRET efficiencies (Ef_A) between PBP2 and RodA variants from spectral FRET measurements. RodA and its variants are fused with mCherry. PBP2 and its variants are fused with mKO. c. Phase contrast images of the complementation of PBP2 variants. The PBP2 temperature sensitive strain LMC582 was transformed with PBP2^WT or PBP2^S330C, and grown in LB medium at 30°C (left panel) and 42°C (right panel) for 2 mass doublings (with 15 μM IPTG induction). Scale bar equals 5 μm. All the results in the figure are representative of at least three independent experiments.