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. 2020 Dec 28;16(12):e1009152. doi: 10.1371/journal.ppat.1009152

Fig 4. Ply mediates aCx43-dependent astrocytic death.

Fig 4

Primary astrocytes derived from wild-type (FL) or aCx43-/- (KO) mice were challenged with wild-type TIGR4 (PN) or an isogenic ply mutant (ply) (a-c), or purified Ply at the indicated concentrations for 60 min (d-g). Samples were fixed and processed for immunofluorescence staining of GFAP (green), F-actin (red) or nuclear DNA using DAPI (cyan). Cbx: challenge in the presence of 100 μM carbenoxolone. Scale bar = 5 μm. a, d, representative micrographs. Arrows point at nuclear shrinkage. b, e, quantification of GFAP debris size. c, g, nuclear size quantification. f, quantification of the number of GFP debris per 104 x μm2 field. Bars: median values. N = 2, > 35 nucleus, > 1500 GFAP debris. Mann-Whitney. **: p < 0.01; ****: p < 0.0001.