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. 2020 Dec 28;16(12):e1009152. doi: 10.1371/journal.ppat.1009152

Fig 5. Cx43 expression confers Ply-mediated cytotoxicity.

Fig 5

Cells were challenged for 90 min with purified Ply (+Ply) at 250 nM or at the indicated concentration. a, wild-type asytrocytes. b-f, HeLa cells or stable transfectants expressing Cx43 (HCx43). Incubation in the presence of: +HEX: hexokinase; + CBX: 100 μM Carbenoxolone. a, b, Cells were fixed and processed for the quantification of nuclear (a) or cell retraction (b). Median values are represented. > 25 cells per sample. Mann-Whitney. ****: p < 0.001. d, > 72 cells per sample. ANCOVA. ****: p < 0.001. c, d, calcein-loaded cells were incubated with 250 nM Ply. c, representative experiment of Ply-induced calcein release. d, rates of calcein decrease determined from linear fits as in “c” (Materials and Methods). Bars: median values. Mann-Whitney. ****: p < 0.001.