Figure 1.

The effects of short-chain fatty acids (SCFA) on the development of the barrier function of 16HBE grown on transwells expressed as a function of time. Barrier function was determined daily before replacement of the medium using trans-epithelial electric resistance (TEER) measurements and were corrected for the background resistance due to the membrane and medium, and membrane surface. Starting from day 4 after seeding, the medium in the basolateral compartment of the transwells was replaced daily by a medium containing different concentrations of SCFA. (a–c) show the effects of SCFA on the barrier function when treatment was continued until day 10. The symbols shown correspond to the statistical tests performed on the data from measurements on days 5 and 10. All data are expressed as mean ± SEM of n = 3 independently performed experiments. **: p < 0.01 and ****: p < 0.0001 when compared to the control group.