Figure 6.

The effects of SCFA on IL-4-decreased zonula occludens-1 (ZO-1) expression in 16HBE. (a) shows that when confluent 16HBE were treated with or without IL-4 (50 ng/mL) for 6, 24 and 48 h and ZO-1 expression was decreased by IL-4 stimulation. (b) shows that treatment with 10 mM acetate, 0.5 mM propionate and 1 mM butyrate for 24 h restored ZO-1 expression in 24 h IL-4 stimulated 16HBE. GAPDH is shown as a control. n = 3 independently performed experiments. *: p < 0.05 when compared to control, #: p < 0.05 when compared to the IL-4 solely stimulated group.