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. 2020 Dec 28;16(12):e1008603. doi: 10.1371/journal.pgen.1008603

Fig 3. Rad51, Rad52 and Rad53 are required for telomere folding.

Fig 3

A. SMC6, SMC1 and SMC4 were tagged with an auxin-inducible degron (AID*) and a 9MYC-tag. Western blots were performed to detect protein levels after treatment with 1 mM IAA for 1 h. B. Telo-3C analysis after depletion of SMC complex members for 1 h with 1 mM IAA. Mean +/- SD of 3 independent experiments. Adjusted p-values were obtained from two-way ANOVA with Tukey’s multiple comparisons test (*p ≤ 0.05, n.s., not significant). C. Telo-3C analysis of rif1 and rif2 mutants. Mean +/- SD of 3 independent experiments. Adjusted p-values were obtained from one-way ANOVA with Dunnett’s multiple comparisons test (n.s., not significant). D. Telo-3C analysis of rad51 and rad52 mutants. Mean +/- SD of 5 independent experiments. Adjusted p-values were obtained from one-way ANOVA with Dunnett’s multiple comparisons test (****p ≤ 0.0001). E. RAD53 was tagged with an auxin-inducible degron (AID*) and a 9MYC-tag. Western blots were performed to detect protein levels after treatment with 1 mM IAA for 2 h. F. Telo-3C analysis after depletion of Rad53 for 2 h with 1 mM IAA. Mean +/- SD of 3 independent experiments. Adjusted p-values were obtained from two-way ANOVA with Tukey’s multiple comparisons test (*p ≤ 0.05). G. Telo-3C analysis of sml1 and sml1 mec1 mutants. Mean +/- SD of 4 independent experiments. Adjusted p-values were obtained from one-way ANOVA with Tukey’s multiple comparisons test (n.s., not significant). For all Telo-3C results, the interaction frequencies between primers P5 and P6 are shown. All interaction frequencies were normalized to a control qPCR product. Relative interaction frequencies were calculated by setting the normalized interaction frequency of the respective wt sample or the untreated sample from each genotype to 1. IAA = Indole-3 acetic acid.