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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Mol Cancer Res. 2019 Apr 9;17(7):1571–1581. doi: 10.1158/1541-7786.MCR-18-1044

Figure 5. p21 knockout inhibits LEF1, but not TCF4, expression.

Figure 5.

(A, B) PyMT1 and p21KO1 cell lines that were untreated or treated with Wnt3a for 18 hrs, were tested by Taqman qRT-PCR for expression of LEF1 and TCF4 mRNA, or for (C) Axin 2 or LRP6 mRNA expression. (D) Two PyMT (PyMT 1–2) and p21KO (p21KO1–2) cell lines that were untreated or treated with Wnt3a for 18hrs were immunoblotted for levels of p-LRP6, LRP6, and Actin. Densitometry of LRP6 and p-LRP6 immunoblots did not reveal any significant differences between PyMT and p21KO cells (Supplemental Fig. 1B–C).