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. 2021 Jan 8;7(2):eabe2086. doi: 10.1126/sciadv.abe2086

Fig. 2. Free-running rhythms in B. subtilis following entrainment in temperature cycles.

Fig. 2

Bioluminescence of PytvA::lux in constant darkness at 25.5°C following 5 days of entrainment in temperature cycles (CW indicates the cold/warm cycle of 12 hours at 25.5°C/12 hours at 28.5°C) is shown. A free-running rhythm is observed in nutrient sporulation medium (NSMP) lacking glucose (A). The detrended data are plotted as means ± SD. The calculated period length of PytvA::lux expression shown in (A) is plotted in (B), where individual data points (N = 8) are shown along with the median and interquartile range. No free-running rhythm is observed in NSMP medium containing glycerol (N = 40) (C) or glucose (N = 15) (D) as a carbon source. See also table S1.