Table 1. Measured and calculated seawater parameters (±SE) from the culturing experiments (S, salinity; T, temperature; pH (NBS scale); DIC, dissolved inorganic carbon; Alkalinity, total alkalinity; pH on total scale; [CO32−], carbonate ion; [HCO3−], bicarbonate ion; [CO2], partial pressure of CO2; ΩA, saturation state of seawater with respect to aragonite).
Experiment | Seawater measured parameters | |||||
δ11Bsw* (‰) |
S (psu) |
T (°C) |
pH (NBS scale) |
DIC (μmol/kg-seawater) |
Alkalinity (μmol/kg-seawater) |
|
Exp1 (400 ppm, 28°C) | 41.02 ± 0.49 | 34.9 ± 0.1 | 28.29 ± 0.01 | 8.27 ± 0.01 | 2480 ± 40 | 2915 ± 49 |
Exp2 (400 ppm, 31°C) | 40.17 ± 0.33 | 35.6 ± 0.1 | 31.72 ± 0.06 | 8.24 ± 0.01 | 2350 ± 44 | 2774 ± 54 |
Exp3 (1000 ppm, 28°C) | 41.36 ± 0.13 | 35.4 ± 0.1 | 27.88 ± 0.02 | 8.04 ± 0.01 | 2645 ± 44 | 2939 ± 46 |
Exp4 (1000 ppm, 31°C) | 41.93 ± 0.20 | 36.0 ± 0.1 | 30.83 ± 0.02 | 8.12 ± 0.01 | 2755 ± 37 | 3083 ± 40 |
Exp5 (2800 ppm, 28°C) | 40.97 ± 0.15 | 35.8 ± 0.1 | 28.17 ± 0.02 | 7.62 ± 0.01 | 2861 ± 82 | 2907 ± 81 |
Exp6 (2800 ppm, 31°C) | 41.35 ± 0.12 | 35.7 ± 0.1 | 30.93 ± 0.02 | 7.69 ± 0.01 | 3223 ± 57 | 3290 ± 56 |
Experiment | Seawater calculated parameters | |||||
pCO2 (ppmv) |
pH** (total scale) |
[CO32−] (μmol/kg-seawater) |
[HCO3−] (μmol/kg-seawater) |
[CO2] (μmol/kg-seawater) |
ΩA | |
Exp1 (400 ppm, 28°C) | 466 ± 8 | 8.14 ± 0.01 | 334 ± 9 | 2133 ± 33 | 12.3 ± 0.2 | 5.38 ± 0.14 |
Exp2 (400 ppm, 31°C) | 499 ± 9 | 8.09 ± 0.01 | 320 ± 11 | 2017 ± 36 | 12.3 ± 0.3 | 5.20 ± 0.18 |
Exp3 (1000 ppm, 28°C) | 925 ± 15 | 7.90 ± 0.01 | 217 ± 6 | 2424 ± 35 | 24.6 ± 0.4 | 3.48 ± 0.10 |
Exp4 (1000 ppm, 31°C) | 885 ± 12 | 7.97 ± 0.01 | 265 ± 4 | 2468 ± 35 | 22.0 ± 0.4 | 4.28 ± 0.07 |
Exp5 (2800 ppm, 28°C) | 2807 ± 120 | 7.49 ± 0.01 | 90 ± 3 | 2697 ± 77 | 74.3 ± 3.2 | 1.44 ± 0.04 |
Exp6 (2800 ppm, 31°C) | 3194 ± 136 | 7.55 ± 0.01 | 113 ± 4 | 3030 ± 54 | 79.5 ± 3.4 | 1.83 ± 0.06 |
*δ11Bsw-based average of δ11Bsw1 and δ11Bsw2 (see table S5) for each condition.
**pH on total scale recalculated on the basis of pH measured on NBS scale during experiment.