Table 3. Measured values of δ11B and elemental ratios (B/Ca) within the skeletons of S. pistillata and corresponding reconstructed parameters of the calcifying fluid (pHcf, [CO32−]cf, and DICcf).
| Culture conditions | Analytical results | |||||||||
| pCO2sw | T | pHsw | Tank | δ11Bc1 (±2 SD) | δ11Bc2 (±2 SD) | δ11Baverage (±2 SD) | B/Ca (±2 SD) | pHcf | [CO32−]cf (±1 SE) | DICcf (±1 SE) |
| (ppm) | (°C) | (total scale) | (‰) | (‰) | (‰) | (μmol/mol) | (total scale) | (μmol/kg) | (μmol/kg) | |
| Stylophora pistillata | ||||||||||
| 466 | 28 | 8.14 | 2 | 23.20 ± 0.30 | 23.21 ± 0.30 | 23.20 ± 0.21 | 470 ± 11 | 8.31 | 888 ± 70 | 4464 ± 191 |
| 466 | 28 | 8.14 | 2 | 455 ± 11 | 918 ± 71 | 4614 ± 194 | ||||
| 466 | 28 | 8.14 | 2 | 459 ± 11 | 909 ± 70 | 4584 ± 190 | ||||
| 466 | 28 | 8.14 | 4 | 21.85 ± 0.30 | 21.63 ± 0.30 | 21.74 ± 0.21 | 527 ± 11 | 8.21 | 659 ± 62 | 4021 ± 166 |
| 466 | 28 | 8.14 | 1 | 23.95 ± 0.26 | 23.95 ± 0.26 | 432 ± 11 | 8.36 | 1050 ± 78 | 4815 ± 216 | |
| 466 | 28 | 8.14 | 2 | 24.94 ± 0.26 | 24.94 ± 0.26 | 480 ± 11 | 8.43 | 1048 ± 70 | 4291 ± 181 | |
| 499 | 31 | 8.09 | 2 | 18.09 ± 0.26 | 18.09 ± 0.26 | 378 ± 11 | 7.92 | 553 ± 84 | 5283 ± 284 | |
| 499 | 31 | 8.09 | 4 | 21.62 ± 0.09 | 21.62 ± 0.09 | 536 ± 11 | 8.21 | 718 ± 54 | 3850 ± 163 | |
| 499 | 31 | 8.09 | 1 | 23.48 ± 0.09 | 23.48 ± 0.09 | 513 ± 11 | 8.34 | 934 ± 59 | 3968 ± 163 | |
| 499 | 31 | 8.09 | 1 | 526 ± 11 | 983 ± 63 | 4179 ± 184 | ||||
| 499 | 31 | 8.09 | 4 | 20.93 ± 0.26 | 20.93 ± 0.26 | 411 ± 11 | 8.16 | 851 ± 79 | 5046 ± 231 | |
| 499 | 31 | 8.09 | 3 | 19.62 ± 0.09 | 19.62 ± 0.09 | 462 ± 11 | 8.06 | 616 ± 63 | 4451 ± 203 | |
| 499 | 31 | 8.09 | 1 | 22.04 ± 0.09 | 22.04 ± 0.09 | 557 ± 11 | 8.24 | 728 ± 53 | 3692 ± 160 | |
| 499 | 31 | 8.09 | 1 | 473 ± 11 | 859 ± 62 | 4351 ± 187 | ||||
| 925 | 28 | 7.90 | 2 | 23.26 ± 0.36 | 23.26 ± 0.36 | 419 ± 11 | 8.29 | 980 ± 77 | 5097 ± 217 | |
| 925 | 28 | 7.90 | 4 | 22.70 ± 0.26 | 22.70 ± 0.26 | 440 ± 11 | 8.25 | 870 ± 67 | 4885 ± 207 | |
| 925 | 28 | 7.90 | 1 | 22.25 ± 0.26 | 22.25 ± 0.26 | 451 ± 11 | 8.22 | 800 ± 64 | 4770 ± 204 | |
| 925 | 28 | 7.90 | 3 | 23.63 ± 0.26 | 23.63 ± 0.26 | 449 ± 11 | 8.32 | 958 ± 67 | 4747 ± 200 | |
| 925 | 28 | 7.90 | 2 | 23.32 ± 0.26 | 23.32 ± 0.26 | 485 ± 11 | 8.30 | 855 ± 62 | 4403 ± 184 | |
| 885 | 31 | 7.97 | 3 | 23.01 ± 0.21 | 22.79 ± 0.21 | 22.90 ± 0.15 | 8.19 | |||
| 885 | 31 | 7.97 | 1 | 23.21 ± 0.39 | 23.21 ± 0.39 | 417 ± 11 | 8.21 | 920 ± 82 | 5038 ± 220 | |
| 885 | 31 | 7.97 | 3 | 19.64 ± 0.26 | 19.64 ± 0.26 | 378 ± 11 | 7.91 | 541 ± 79 | 5346 ± 286 | |
| 885 | 31 | 7.97 | 4 | 19.76 ± 0.26 | 19.76 ± 0.26 | 595 ± 11 | 7.92 | 353 ± 50 | 3408 ± 161 | |
| 885 | 31 | 7.97 | 1 | 17.49 ± 0.26 | 17.49 ± 0.26 | 394 ± 11 | 7.65 | 251 ± 73 | 4347 ± 474 | |
| 885 | 31 | 7.97 | 2 | 23.27 ± 0.39 | 23.27 ± 0.39 | 417 ± 11 | 8.22 | 929 ± 84 | 5036 ± 219 | |
| 2807 | 28 | 7.49 | 3 | 22.11 ± 0.39 | 22.11 ± 0.39 | 384 ± 11 | 8.23 | 983 ± 86 | 5622 ± 251 | |
| 2807 | 28 | 7.49 | 3 | 21.90 ± 0.39 | 21.90 ± 0.39 | 377 ± 11 | 8.22 | 968 ± 86 | 5726 ± 257 | |
| 2807 | 28 | 7.49 | 4 | 21.51 ± 0.39 | 21.51 ± 0.39 | 357 ± 11 | 8.19 | 969 ± 94 | 6046 ± 287 | |
| 2807 | 28 | 7.49 | 4 | 21.40 ± 0.39 | 21.40 ± 0.39 | 355 ± 11 | 8.18 | 958 ± 95 | 6095 ± 286 | |
| 3194 | 31 | 7.55 | 4 | 21.74 ± 0.39 | 21.74 ± 0.39 | 410 ± 11 | 8.15 | 819 ± 81 | 5101 ± 231 | |
| 3194 | 31 | 7.55 | 2 | 22.75 ± 0.39 | 22.75 ± 0.39 | 425 ± 11 | 8.22 | 903 ± 79 | 4895 ± 214 | |
| 3194 | 31 | 7.55 | 3 | 22.62 ± 0.36 | 22.62 ± 0.36 | 408 ± 11 | 8.21 | 928 ± 78 | 5106 ± 226 | |
| 3194 | 31 | 7.55 | 3 | 22.05 ± 0.36 | 22.05 ± 0.36 | 419 ± 11 | 8.17 | 836 ± 78 | 4984 ± 218 | |
| 3194 | 31 | 7.55 | 1 | 19.15 ± 0.39 | 19.15 ± 0.39 | 342 ± 11 | 7.92 | 605 ± 93 | 5888 ± 330 | |
| 3194 | 31 | 7.55 | 2 | 22.07 ± 0.28 | 22.14 ± 0.28 | 22.10 ± 0.20 | 455 ± 11 | 8.17 | 773 ± 61 | 4585 ± 202 |
pCO2sw, seawater pCO2 of the culture treatment.
T, seawater temperature of the culture treatment.
pHsw, seawater pH of the culture treatment.
Tank, tank number where the sample was cultured.
δ11Bc1, first measurement of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).
δ11Bc2, duplicate analysis of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).
δ11Baverage, average of δ11Bc1 and δ11Bc2 or δ11Bc1 if only one measurement was performed. When two measurements were carried out uncertainty was calculated based on uncertainties of δ11Bc1: and δ11Bc2 (Δa = √ (Σi(Δai)2)).
B/Ca measured for the sample. Uncertainty is based 2 SD on the reproducibility of the CamWuellestorfi (table S4).
pHcf calculated from δ11B using (8) based on Monte Carlo simulation.
[CO32−]cf calculated from B/Ca using (8).
DICcf calculated from δ11B and B/Ca using (8).
Uncertainties for [CO32−]cf and DICcf calculated using (8), Δ (1 SE) = √ (Σi(Δsysi+Δnonsysi)2), xuncertainty = Δnonsys (1 SE).Additional data not used in this study (Li/Ca, Mg/Ca and Sr/Ca) are presented in table S7 in the Supplementary Materials.