Fig. 1. Colloidally stable SPIONs consisting of a PEGsilane/APS coating backfilled with additional PEG.
(A) Intensity weighted distribution of backfilled PEG-coated SPIONs in 1× PBS over 28 days. a.u., arbitrary units. (B) Intensity weighted distribution of backfilled PEG-coated SPIONs in VS55 (no vitrification). (C) Intensity weighted distribution of backfilled PEG-coated SPIONs in VS55 after vitrification and nanowarming. (D) Arithmetic mean and SD of the DLS hydrodynamic diameter for backfilled SPIONs in 1× PBS and VS55 before and after vitrification and nanowarming over 28 days.