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. 2021 Jan 8;7(2):eabe3005. doi: 10.1126/sciadv.abe3005

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 5 — Hearts were removed from male rats and perfused with Custodiol HTK solution. Then, the heart was perfused with mCPA containing 5 mg_Fe/ml. The perfused heart was submerged in mCPA and vitrified in a mechanical freezer at a cooling rate of 15°C/min, followed by storage in liquid nitrogen for 1 week. Nanowarming was performed in an AMF (42.5 kA/m and 278 kHz, achieving heating rates above the CWR of 50°C/min for VS55. The SPIONs were perfused out of the heart using Custodiol HTK, followed by MPI to quantify the SPIONs remaining in the hearts. LN2, liquid nitrogen.