Author response image 1. Most mutations are found at low frequencies in individual animals.

Frequency of each mutation in individual F0 knockout embryos (samples). Mutations are ranked from the most frequent in the embryo (a 3-bp deletion in gene ta, locus D, embryo 1 was present in 602/605 of the reads) to the least frequent (a 11-bp insertion in gene csnk1db, locus B, embryo 4 was present in 1/3189 of the reads). n = 7015 unique mutations.