Video 5. Optogenetic activation of Amg_C/M-PAG neurons transiently suppresses ultrasonic vocalization (USV) production.

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A male mouse which has ChR2 expressed in Amg_C/M-PAG neurons is shown interacting with and producing USVs directed at a female social partner. Optogenetic activation of these neurons with pulses of blue light transiently suppresses USV production without suppressing non-vocal courtship behavior. Video is shown at the top, a spectrogram (bottom) showing the optogenetically elicited USVs is synchronized to the video, and pitch-shifted audio (80 kHz to 5 kHz transformation) is included to place the USVs within the human hearing range.