Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | MYC | NA | UniProt ID: P01106 | |
| Gene (Homo sapiens) | HCFC1 | NA | UniProt ID: P51610 | |
| Strain, strain background (Escherichia coli) | Rosetta (DE3) Competent Cells | Millipore | Cat#: 70954 | |
| Strain, strain background (Escherichia coli) | XL1-Blue Competent Cells | Agilent Technologies | Cat#: 200249 | |
| Cell line (Homo sapiens) | Ramos | ATCC | Cat#: CRL-1596; RRID:CVCL_0597 | |
| Cell line (Homo sapiens) | Ramos Cre-ERT2 | Thomas et al., 2019 | NA | |
| Cell line (Homo sapiens) | Ramos Cre-ERT2 MYC-WT | Thomas et al., 2019 | NA | |
| Cell line (Homo sapiens) | Ramos Cre-ERT2 MYC-Δ264 | Thomas et al., 2019 | NA | |
| Cell line (Homo sapiens) | Ramos Cre-ERT2 MYC-4A | This paper | NA | See section ‘Generation of switchable MYC allele Ramos cell lines’ |
| Cell line (Homo sapiens) | Ramos Cre-ERT2 MYC-VP16 HBM | This paper | NA | See section ‘Generation of switchable MYC allele Ramos cell lines’ |
| Cell line (Homo sapiens) | Ramos FKBPFV-HCF-1N | This paper | NA | See section ‘Generation of dTAG Ramos cell lines’ |
| Antibody | Rabbit anti-HCF-1C polyclonal | Bethyl Laboratories | Cat#: A301-399A; RRID:AB_961012 | Western blotting (1:1000) |
| Antibody | Rabbit anti-HCF-1N polyclonal | Machida et al., 2009 | NA | Western blotting (1:10,000), ChIP (6 μl) |
| Antibody | Rabbit anti-c-MYC (Y69) monoclonal | Abcam | Cat#: ab32072; RRID:AB_731658 | Western blotting (1:10,000), IP (2 μg) |
| Antibody | Rabbit anti-HA (C29F4) monoclonal | Cell Signaling Technology | Cat#: 3724; RRID:AB_1549585 | Western blotting (1:4000), ChIP (5 μl), IF (1:500) |
| Antibody | Rabbit anti-WDR5 (D9E1I) monoclonal | Cell Signaling Technology | Cat#: 13105; RRID:AB_2620133 | Western blotting (1:1000) |
| Antibody | Rabbit anti-IgG polyclonal | Cell Signaling Technology | Cat#: 2729; RRID:AB_1031062 | IP (2 μg), ChIP (0.8 μg) |
| Antibody | Mouse anti-FLAG (M2) monoclonal (HRP) | Sigma-Aldrich | Cat#: A8592; RRID:AB_439702 | Western blotting (1:10,000 or 1:50,000) |
| Antibody | Mouse anti-T7 monoclonal (HRP) | Millipore | Cat#: 69048; RRID:AB_11212778 | Western blotting (1:10,000) |
| Antibody | Mouse anti-GAPDH (GA1R) monoclonal (HRP) | Thermo Fisher Scientific | Cat#: MA5-15738-HRP; RRID:AB_2537659 | Western blotting (1:50,000) |
| Antibody | Rabbit anti-GAPDH (D16H11) monoclonal (HRP) | Cell Signaling Technology | Cat#: 8884; RRID:AB_11129865 | Western blotting (1:2500) |
| Antibody | Rabbit anti-α-Tubulin (11H10) monoclonal (HRP) | Cell Signaling Technology | Cat#: 11H10; RRID:AB_10695471 | Western blotting (1:5000) |
| Antibody | Rabbit anti-histone H3 (D1H2) XP monoclonal (HRP) | Cell Signaling Technology | Cat#: 12648; RRID:AB_2797978 | Western blotting (1:5000) |
| Antibody | Goat anti-rabbit IgG polyclonal (HRP) | Thermo Fisher Scientific | Cat#: 31463; RRID:AB_228333 | Western blotting (1:5000) |
| Antibody | Mouse anti-rabbit IgG monoclonal, light chain specific (HRP) | Jackson ImmunoResearch Labs | Cat#: 211-032-171; RRID:AB_2339149 | Western blotting (1:5000) |
| Antibody | Goat anti-rabbit IgG polyclonal (Alexa Fluor 594) | Thermo Fisher Scientific | Cat#: A11012; RRID:AB_2534079 | IF (1:350) |
| Other | Anti-FLAG M2 affinity gel | Sigma-Aldrich | Cat#: A2220; RRID:AB_10063035 | IP (20 μl bed volume) |
| Other | Protein A Agarose | Roche | Cat#: 11134515001 | IP/ChIP (20 μl bed volume) |
| Recombinant DNA reagent | pCRIS-mCherry-FLAG-dTAG-HCFC1 | This paper | NA | See section ‘Generation of dTAG Ramos cell lines’ |
| Recombinant DNA reagent | pGuide-HCFC1-N | This paper | NA | See section ‘Generation of dTAG Ramos cell lines’ |
| Recombinant DNA reagent | pX330-U6-Chimeric_BB-CBh-hSpCas9 | Addgene | Cat#: 42230; RRID:Addgene_42230 | |
| Recombinant DNA reagent | MYC-WT targeting vector | Thomas et al., 2019 | NA | |
| Recombinant DNA reagent | MYC-4A targeting vector | This paper | NA | See section ‘Generation of switchable MYC allele Ramos cell lines’ |
| Recombinant DNA reagent | MYC-VP16 HBM targeting vector | This paper | NA | See section ‘Generation of switchable MYC allele Ramos cell lines’ |
| Recombinant DNA reagent |
pGuide-MYC1 | This paper | NA | See section ‘Generation of switchable MYC allele Ramos cell lines’ |
| Recombinant DNA reagent | pFLAG-C2 | Thomas et al., 2015 | NA | |
| Recombinant DNA reagent | pFLAG-MYC WT | Thomas et al., 2015 | NA | |
| Recombinant DNA reagent | pFLAG-MYC 4A | This paper | NA | See section ‘Transient transfection, western blotting, and immunoprecipitation’ |
| Recombinant DNA reagent | pFLAG-MYC H307G | This paper | NA | See section ‘Transient transfection, western blotting, and immunoprecipitation’ |
| Recombinant DNA reagent | pFLAG-MYC VP16 HBM | This paper | NA | See section ‘Transient transfection, western blotting, and immunoprecipitation’ |
| Recombinant DNA reagent | pFLAG-MYC VP16 HBM H307G | This paper | NA | See section ‘Transient transfection, western blotting, and immunoprecipitation’ |
| Recombinant DNA reagent | pFLAG-MYC WBM | Thomas et al., 2015 | NA | |
| Recombinant DNA reagent | pSUMO-MYC WT-FLAG | Thomas et al., 2015 | NA | |
| Recombinant DNA reagent | pSUMO-MYC 4A-FLAG | This paper | NA | See section ‘In vitro binding assays’ |
| Recombinant DNA reagent | pSUMO-MYC VP16 HBM-FLAG | This paper | NA | See section ‘In vitro binding assays’ |
| Recombinant DNA reagent | pT7-IRES His-T7-HCF-1VIC | This paper | NA | See section ‘In vitro binding assays’ |
| Recombinant DNA reagent | pRSET-6XHis-MYC WT | Farina et al., 2004 | NA | |
| Recombinant DNA reagent | pRSET-6XHis-MYC 4A | This paper | NA | See section ‘Electrophoretic mobility shift assays’ |
| Recombinant DNA reagent | pRSET-6XHis-MYC VP16 HBM | This paper | NA | See section ‘Electrophoretic mobility shift assays’ |
| Commercial assay or kit | Q5 DNA Polymerase | NEB | Cat#: M0491 | |
| Commercial assay or kit | OneTaq DNA Polymerase | NEB | Cat#: M0480 | |
| Commercial assay or kit | Gibson Assembly Cloning Kit | NEB | Cat#: E5510 | |
| Commercial assay or kit | LightShift Chemiluminescent EMSA Kit | Thermo Fisher | Cat#: 20148 | |
| Chemical compound, drug | DMSO | Sigma-Aldrich | Cat#: D2650 | |
| Chemical compound, drug | dTAG-47 | Vanderbilt Institute of Chemical Biology Synthesis Core | ||
| Software, algorithm | PRISM 8 | GraphPad | RRID:SCR_002798 | https://www.graphpad.com/scientific-software/prism/ |
| Software, algorithm | FACSDiva 8.0 | BD Biosciences | RRID:SCR_001456 | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| Software, algorithm | FlowJo | FlowJo | RRID:SCR_008520 | https://www.flowjo.com/ |
| Software, algorithm | MetaboAnalyst 4.0 | Chong et al., 2019 | RRID:SCR_015539 | https://www.metaboanalyst.ca/ |
| Software, algorithm | Metascape | Zhou et al., 2019 | RRID:SCR_016620 | https://metascape.org/ |
| Software, algorithm | DAVID | Huang et al., 2009a, Huang et al., 2009b | RRID:SCR_001881 | https://david.ncifcrf.gov/ |