Table 1. Loss of the MYC–host cell factor (HCF)–1 interaction promotes amino acid accumulation.

Modulating the MYC–HCF-1 interaction alters intracellular amino acid levels. All data are derived from switchable Ramos cells treated with 20 nM 4-OHT for 24 hr. Amino acid levels were measured following separations by hydrophilic interaction liquid chromatography (HILIC). Q-values and fold-changes (FCs) were calculated between the mutants and wild-type (WT). Five biological replicates for WT and VP16 HCF-1-binding motif (HBM) and four biological replicates for 4A were analyzed. Q-value < 0.05 are highlighted in green, FC > 0 in red, and FC < 0 in blue. Confidence levels reflect the confidence in metabolite identification; L1 is validated, L2 is putative, and L3 is tentative. ND = not detected; NS = not significant.

	4A		VP16 HBM		Confidence level
	Q-value	FC	Q-value	FC
Glycine	2.52E-04	1.31	3.11E-05	−1.48	L3
L-alanine	9.97E-05	1.62	NS		L2
L-arginine	2.31E-02	1.18	NS		L1
L-asparagine	6.67E-05	1.5	2.02E-03	−1.23	L2
L-aspartic acid	1.24E-03	−1.37	1.54E-05	2.01	L2
L-cysteine	ND		ND
L-glutamic acid	2.53E-03	1.46	NS		L2
L-glutamine	2.33E-05	1.46	9.38E-05	−1.41	L2
L-histidine	8.70E-05	1.64	NS		L2
L-isoleucine	1.10E-03	1.39	2.36E-02	−1.21	L2
L-leucine	8.63E-04	1.33	2.55E-02	−1.18	L2
L-lysine	1.66E-02	1.19	NS		L2
L-methionine	6.51E-05	1.61	4.39E-03	−1.27	L2
L-phenylalanine	4.71E-04	1.48	3.46E-02	−1.22	L2
L-proline	1.01E-02	1.17	NS		L2
L-serine	7.48E-05	1.59	NS		L1
L-threonine	9.63E-05	1.54	1.25E-02	−1.17	L2
L-tryptophan	1.56E-04	1.64	NS		L1
L-tyrosine	1.22E-04	1.59	1.9E-02	−1.18	L2
L-valine	4.41E-02	1.14	NS		L3