Figure 4.

Degradation of eNAD after a 2-minute contact with freshly dispersed unsorted cells of murine colon tunica muscularis. Scattered plots (mean, SD) of 12 experiments demonstrating the formation of eADPR and eADO from eNAD. Each experiment is performed in one perfusion chamber containing 35,000–62,000 cells collected from 4 mouse colons. S0 is eNAD substrate in the absence of cells. S2’ is sample collected after contact of eNAD with cells. Asterisks denote significant difference of S2’ vs. S0. ****P < 0.0001. Paired, two-tailed t-test (GraphPadPrism v. 8.3.0., GraphPad Software, Inc, San Diego, CA).