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. 2020 Dec 23;8:825. doi: 10.3389/fcell.2020.00825

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Copyright © 2020 Wen, Xie, Zhang, Li, Li, Zhang, Lu, Zhang, Liu, Liu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MACC1 promotes the proliferation of osteosarcoma cells in vitro. (A) Immunoblot assays and qPCR were performed to measure the protein expression of MACC1 in osteosarcoma (OS) cells following transfection with the indicated plasmids. (B) Colony formation assays were performed to evaluate the difference in proliferative ability between OS cells in the indicated groups. (C) MTT assays were performed to detect the proliferation level in cells transfected with the indicated plasmids. (D) CCK-8 assays were performed to detect the proliferation level in cells transfected with the indicated plasmids. (E) Immunoblot assays were performed to analyze the expression of Ki-67, PCNA, cyclin D2, and cyclin A in OS cells transfected with the indicated plasmids. Quantification of relative protein level were displayed. The results are presented as means ± SEM; ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.