Skip to main content
. 2020 Dec 16;11:597665. doi: 10.3389/fpls.2020.597665

FIGURE 2.

FIGURE 2

Domain analysis and localization of NbHMG1/2a. (A) Predicted domains encoded in NbHMG1/2a: a nuclear localization signal (NLS) between 4 and 56 amino acids (aa), a nucleolar localization signal (NoLS) between 15 and 42 aa, and a DNA-binding domain between 36 and 101 aa. No nuclear export signal was found. (B) NbHMG1/2a localizes in the nucleus. N. benthamina leaves were infiltrated with Agrobacterium carrying pBin61-HA-HMG-mCherry or with control vector pBin61-HA-mCherry. (C) NbHMG1/2a localizes in the nucleolus. N. benthamina leaves were infiltrated with Agrobacterium carrying HA-mCherry and nucleolus-localized fibrillarin fused with eGFP (eGFP-Fib), or with HA-mCherry-HMG and eGFP-Fib. DAPI was used for nuclear staining. Images were taken 3 days after infiltration, and bars represent a measurement scale of 20 and 10 μm for the nucleus and nucleolus, respectively. The experiment was repeated three times with similar results and representative images are shown.