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. 2020 Dec 16;11:597665. doi: 10.3389/fpls.2020.597665

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Copyright © 2020 Alazem, He, Chang, Cheng and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of silencing NbHMG1/2a on plant growth and resistance to BaMV. (A) Schematic illustration of our VIGS experimental design. The first three leaves of N. benthamiana plants were infiltrated with the silencing vector TRV carrying the NbHMG1/2a fragment or control mCherry fragment. Eight days later, leaves 7 and 8 were infiltrated with pBin61 carrying BaMV (pKBG). Inoculated leaves (leaves 7 and 8) and systemic leaves (leaves 11 and 12) were collected at 3, 4, and 6 days after BaMV infection (dpi). (B) Phenotype of NbHMG1/2a-silenced plants. Plants infiltrated with TRV-mCherry, TRV-HMG, and TRV-PDS. Photos were taken 8 days after agroninfiltration with TRVs. Upper and middle panel show plant height and spread, and lower panel shows leaf size. (C,D) RNA blot of BaMV in the inoculated leaves (C) and systemic leaves (D) of control plants (infiltrated with TRV-mCherry) and NbHMG1/2a-silenced plants (HMG-VIGS). The second panel represents rRNA levels as internal control. The third panel shows NbHMG1/2a levels in the control and silenced plants. eIF1α was used as an internal control. Values represent average accumulation of BaMV genomic RNA from three biological replicates ± standard deviation. Lower panels represent protein blots of BaMV capsid protein (CP) (25 kDa) and actin in the control and silenced plants.