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. 2020 Dec 16;11:597665. doi: 10.3389/fpls.2020.597665

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Copyright © 2020 Alazem, He, Chang, Cheng and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of NbHMG1/2a enhances systemic movement of BaMV. (A) Twenty-four-day-old N. benthamiana plants were co-agroinfiltrated with pKBG and pBin-HA-mCherry or pBin-HA-mCherry-HMG. Samples were collected from inoculated and systemic leaves at 4, 5, and 6 dpi. Upper panels are Northern blots for BaMV, with rRNA used as a loading control. Lower panels are protein blots of BaMV CP and HA-tag to detect HA-mCherry (30 kDa) and HA-mCherry-HMG (45 kDa). Ponceau S was used as a loading control. The experiment was conducted with three biological replicates, which generated similar results. Values below Northern blots represent average accumulation of BaMV genomic RNA from three biological replicates ± SD. (B) Relative expression of endogenous NbHMG1/2a and the overexpressed gene NbHMG1/2a-mCherry in inoculated and systemic leaves of NbHMG1/2a-overexpressing plants based on RTqPCR. According to one-sided student t-test, asterisk indicates significant difference for the expression of NbHMG1/2a-mCherry relative to the endogenous NbHMG1/2a, with ** representing P < 0.01. Actin was used as an internal control. Error bars represent the standard deviation from three biological replicates.