FIGURE 5.

Relative expression of host genes involved in BaMV movement in NbHMG1/2a- knockdown and -overexpressing plants. Transcript levels of RAB-GAP1 (A,E), CK2α (B,F), STKL (C,G), and TRXh2 (D,H) in N. benthamiana plants infected with pKBG or pKn and upon HMG being knocked-down or overexpressed, respectively. Expression level was measured in the BaMV-inoculated leaves at 6 dpi in both HMG-silenced and HMG-overexpressed plants. Actin was used as an internal control. Data represent the mean ± SD from three biological replicates. One-sided Student’s t-tests were performed to determine significant differences. Asterisks indicate significant differences relative to mock lines (infiltrated with pKn and pBin-mCherry), with * representing P < 0.05.