Figure 1.

Chondrosarcoma 14 day alginate spheroid cultures were assessed for (A) Spheroids presented chondrosarcoma-like histology (as seen by HandE) and were positive for chondrosarcoma extracellular matrix components such as glycosaminoglycans (toluidine and alcian blue staining) (B) Collagen II expression was present in all three cell lines and (C) expression levels remained stable or increased over time (n ≥ 3). Scale bar = 20 μm.