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. 2020 Dec 23;11:604690. doi: 10.3389/fpls.2020.604690

Figure 12.

Figure 12

Analysis of the function of soybean GmGRAS37 under normal conditions and drought and salt stresses. (A–C) Phenotypes of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control (expressing the pCAMBIA3301 empty vector), and GmGRAS37-overexpression (35S::GRAS37) plants under normal conditions (A), drought stress (B), and salt stress (C). (D–F) The roots of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under normal conditions (A), drought stress (B), and salt stress (C). (G,I) Trypan blue staining of leaves of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought (G) and salt stress (I); the dead cells can be strained, but living cells cannot. (H,J) Nitroblue tetrazolium (NBT) staining of the leaves of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought (H) and salt stress (J). The intensity of color indicates the concentration of O2− in the leaves. (K–M) CAT (K), POD (L), and SOD (M) activities of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought conditions. (N,O) Chlorophyll content (N) and MDA content (O) of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought conditions. (P,Q) The fresh weights of the aerial parts (P) and roots (Q) of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought conditions. (R) Relative electrical conductivity of transgenic soybean hairy root composite GRAS37-RNAi, EV-Control, and 35S: GRAS37 plants under drought conditions. Vertical bars indicate ±SD of three replicates. ∗ (p < 0.05) and ∗∗ (p < 0.01) indicate significant differences determined by Student’s t-test.