FIGURE 5.
EMP25 is required for the splicing of nad5 introns. (A) Schematic diagram of the maize mitochondrial nad5 gene and RT-PCR analysis of intron splicing efficiency in the WT and emp25. Introns 1 and 4 are cis-splicing introns. Introns 2 and 3 are trans-splicing introns. The expected amplification products using different primer pairs are indicated. The arrows indicate the unspliced fragments of introns 1 and 4 (cis-splicing). (B) Quantitative RT-PCR (qRT-PCR) analysis of the 22 group II intorns in maize mitochondrial genes in two alleles of emp25. Values represent the mean and standard deviation of three biological replicates.
