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. 2020 Dec 23;11:608550. doi: 10.3389/fpls.2020.608550

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Copyright © 2020 Xiu, Peng, Wang, Yang, Sun, Wang, Cao, Jiang, Wang, Chen and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Defect in the complex assembly and activity in emp24 and emp25. (A,B) The blue native polyacrylamide gel electropheresis (BN-PAGE) gel was stained with Coomasssie Brilliant Blue (CBB). In-gel NADH dehydrogenase activity assay of complex I used dihydrolipoamide dehydrogenase (DLDH) as a loading control. C I: Complex I; C III: Complex III; C V: Complex V; *: sub-complex I detected in mutant. (C) Crude mitochondrial extracts from endosperm and embryo of emp25 with sibling WT kernels were used for Western blot analysis with antibodies against Nad9, Cyt c1, Atpase, Cox2, and AOX. CBB staining was used as a loading control.