Figure 1.

Endothelial cells enhance the proliferation of prostate cancer cells with or without docetaxel treatment. (A) This picture showed the construction of the co-culture system by using a 0.4 μm pore Transwell plate. (B, C) CCK8 assays revealed that the co-culture group with human umbilical vein endothelial cells (HUVEC) increased the growth rate of 22Rv1 and C4–2B compared with the respective monoculture group. (D, E) EdU assays demonstrated proliferation differences between 22Rv1 cultured alone and 22Rv1 co-cultured with HUVEC, C4–2B cultured alone, and C4–2B co-cultured with HUVEC. (F, G) Cell viability of 22Rv1 and C4–2B (cultured with or without HUVEC) treated with docetaxel at the indicated concentrations for 48 h. The data represent the mean ± SD and differences were tested by the Student’s t-test; *P < 0.05; **P < 0.01.