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. 2020 Dec 16;10:584505. doi: 10.3389/fonc.2020.584505

Figure 5.

Figure 5

ETS related gene (ERG) is involved in the regulation of the Akt/mTOR signaling pathway in prostate cancer cells. (A) Western blotting analysis of total Akt, phosphorylated Akt (p-Akt), total mTOR, phosphorylated mTOR (p-mTOR) proteins in C4–2B and C4-2B-ERG cells. (B) C4–2B cells were co-cultured with human umbilical vein endothelial cells (HUVEC) for 48 h, and total Akt, phosphorylated Akt (p-Akt), total mTOR, phosphorylated mTOR (p-mTOR) proteins were measured subsequently by western blotting. (C) C4–2B cells were treated with 50 ng/ml FGF2 for 48 h, western blotting analysis was performed subsequently for total Akt, phosphorylated Akt (p-Akt), total mTOR, phosphorylated mTOR (p-mTOR) proteins. (D) The apoptosis rates of C4-2B-ERG cells were determined by flow cytometry following docetaxel treatment with or without Akt inhibitor. (E) Cell viability of C4-2B-ERG cells treated with docetaxel at the indicated doses with or without Akt inhibitor for 48h. Data were presented as mean ± SD; **P < 0.01; ***P < 0.001.