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. 2020 Dec 18;12:601965. doi: 10.3389/fnagi.2020.601965

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Copyright © 2020 Liu, Chen, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-PCR validation assay of hsa_circ_0003391 expression in peripheral blood of AD patients. Genomic locus of hsa_circ_0003391 (A). RT-PCR products with divergent primers (B) of hsa_circ_0003391 verified that hsa_circ_0003391 naturally exists in the peripheral blood of patients with AD (C). Sanger sequencing of hsa_circ_0003391 shows the backsplice junction (D). RT-qPCR was used to detect the stability of hsa_circ_0003391 in five whole blood cell samples from patients with AD at three different temperatures (25, 4, and −20°C) for 0, 2, 6, and 12 h (E).