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. 2020 Dec 16;8:604123. doi: 10.3389/fbioe.2020.604123

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Copyright © 2020 Vonbrunn, Mueller, Pichlsberger, Sundl, Helmer, Wallner, Rinner, Tuca, Kamolz, Brislinger, Glasmacher and Lang-Olip.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical staining of hAMSC-seeded carriers to detect cell adhesion and migration. Matriderm and PCL/PLA were cultured with hAMSCs suspended in EGM-2 (uncoated) or Matrigel (coated) for (A) 3 days and (B) 8 days. The hAMSCs were visualized with anti-vimentin (brown color). The dotted line marks the side of Matriderm and PCL/PLA that was seeded with cells. Matrigel (M) was applied to the surface of the coated Matriderm and PCL/PLA layers. w-m, wide-meshed PCL/PLA layer; d-m, dense-meshed PCL/PLA layer.