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Serial assessment of substrate utilization in HP murine hearts. Real-time quantitative PCR analyses for the gene expression of (A) pdk4, (B) glut4, (C) mcad, (D) mcd, (E) pparα, and (F) pgc1α. Representative images (G) and densitometric assessment (H,I) of cardiac p-AMPK and p-ACC as acquired by Western blot analysis. *P < 0.05 vs. Baseline; †P < 0.05 vs. TAC 3d; ‡P < 0.05 vs. De-TAC 4d.
