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. 2020 Dec 7;8:601216. doi: 10.3389/fbioe.2020.601216

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Copyright © 2020 Donati, Pomili, Boselli and Pompa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro colorimetric assay using MGNPs suspended in saliva: (A) schematics of the mechanisms involved; (B) LSPR Δλ_max evolution over time related to the reshaping in saliva and in water (the two model platforms performance are compared at their own Br^– optimized conditions while involving the same particles number, and H₂O₂ final concentration, 2 mM). TEM micrographs related to the MGNPs before (C) and after (D) the assay with non-supplemented saliva (blue) and with glucose supplement to simulate hyperglycemia condition (red). (E) Statistical TEM analysis measuring tips (numbers and lengths) and “core” dimensional variation between control and sample nanostructures (p < 0.001 in all cases). Statistical significance was determined by Mann-Whitney test (***p < 0.001). See experimental details in Supplementary Material.