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. 2020 Dec 10;11:592818. doi: 10.3389/fendo.2020.592818

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Copyright © 2020 Duan, Ge, Jiang, Zhang, Zhao, Li, Zhang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diphyllin promotes differentiation and thermogenesis in primary brown (A–I) and beige (J–R) adipocytes. (A, J) Oil red O staining of differentiated brown and beige adipocytes treated with diphyllin, N=4. (B, K) Quantitative statistics of oil red O staining. (C, L) The protein levels in brown adipocytes after the differentiation test; (D, M) The relative gene mRNA levels in brown adipocytes after the differentiation test, n=3. (E, N) The protein levels in brown adipocytes after the thermogenesis test; (F, O) The relative gene mRNA levels in brown adipocytes after the thermogenesis test, n=3. (G, P) Oxygen consumption rates (OCRs) of brown adipocytes after 5 and 10 μM diphyllin treatment for 24 h; (H–I, Q–R). The OCR-related basal metabolic rate and uncoupled oxygen consumption. *p < 0.05, **p < 0.01, and ***p < 0.001.