Table 2.
Primers used for PCR amplification and detection.
| Primers | Primers sequence (5′—3′) | Amplification for |
|---|---|---|
| gidA-F | CGGGATCCATGACACACACATTTGCAGA | gidA gene |
| gidA-R | CGCTCGAGTTAGTGACTGTCCTTTGATTT | |
| mnmE-F | CGGGATCCATGACACACACATTTGCAGA | mnmE gene |
| mnmE-R | CGCTCGAGTTAGTGACTGTCCTTTGATTT | |
| GT-F | GCTTTTGTGGACTTAGAGAAATTGG | Upstream to downstream regions of gidA |
| GT-R | ACGAACCTCAGCCATATTGAGATTT | |
| MT-F | GACAAAATAGCTGGTGTAATAAAG | Upstream to downstream regions of mnmE |
| MT-R | CTGTGTATGAAGGAGTTGAGGC | |
| cps2J-F | TGATAGTGATTTGTCGGGAGGG | cps2J gene |
| cps2J-R | GAGTATCTAAAGAATGCCTATTG | |
| arcA-F | ATGAGAGGCAGGAAAGCGTACGGTC | arcA gene |
| arcA-R | AAGGCATCATGCTCTTTCTGTG | |
| arcB-F | TGTTGGCGGACTACTTGACTG | arcB gene |
| arcB-R | ACGTGCTCCACTTTCTTTCG | |
| arcC-F | ACCCATCGGCTAAAGCACAA | arcC gene |
| arcC-R | TTCCGAATCAGCAGCAAGGT | |
| 16S RNA-F | GTAGTCCACGCCGTAAACG | 16S RNA |
| 16SRNA-R | TAAACCACATGCTCCACCGC |