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. 2020 Dec 3;11:573670. doi: 10.3389/fpls.2020.573670

FIGURE 2.

FIGURE 2

Root colonization of Arabidopsis thaliana by Trichoderma. (A–D) Co-cultivation for 2 days. (A,B) Bright field; (C,D) fluorescence of fungal stain. (E) Confocal images of hyphae inside root hair observed 2 days after co-cultivation. (F) Magnified view of the region enclosed by the small box in (E). (G) Fluorescence signal indicating hyphae (green) and plant cell plasma membrane (red) in (F). (H–K) Co-cultivation for 7 days. (H,I) Bright field; (J,K) fluorescence of fungal stain. Colonized root tissues were stained with WGA Alexa FluorTM 488 conjugate to detect the presence of the fungus, and RH414 was used to visualize the plant plasma membrane. The image shown for the confocal microscopy was chosen from three individual roots of three biological replicates.