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. 2020 Dec 14;8:603598. doi: 10.3389/fbioe.2020.603598

Figure 7.

Figure 7

hBMSC-EV effects on IL-1β-induced activation of Erk1/2, PI3K/Akt p38, TAK1, and NF-κB signaling pathways in OA-CH. The phosphorylation levels of Erk1/2, PI3K/Akt, p38, TAK1, and NF-κB in OA-CH were detected by western blotting and immunofluorescence staining. (A–E) Quantification of protein phosphorylation level (upper panel) and representative western blot images (lower panel) of Erk1/2, P13K/Akt, p38, TAK1, and NF-κB after 30 min of stimulation with Il-1β and EVs. (F–I) Immunofluorescence staining (lower panel) and quantification of protein expression of the phosphorylated forms of Erk1/2, P13K/Akt, p38, and NF-κB (upper panel) in OA-CH after treatment with hBMSC-EVs and/or IL-1β. Scale bar = 100 μm. All values represent mean ± standard deviation. *Significant differences to OA-CH group (control group): *p < 0.05; **p < 0.01; ***p < 0.001; #significant differences between groups: #p < 0.05; ##p < 0.01; one-way ANOVA with Newman–Keuls Multiple Comparison Test; n = 3.