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. 2020 Dec 14;8:603598. doi: 10.3389/fbioe.2020.603598

Table 1.

Primer sequences for qPCR.

Gene Primer sequence
ACAN Fwd: 5′-CTATACCCCAGTGGGCACAT-3′
Rev: 5′-GGCACTTCAGTTGCAGAAGG−3′
BCL2 Fwd: 5′-ATGTGTGTGGAGAGCGTCAA-3′
Rev: 5′-ACAGTTCCACAAAGGCATCC-3′
COMP Fwd: 5′-AGGGAGATCGTGCAGACAA-3′
Rev: 5′-AGCTGGAGCTGTCCTGGTAG-3′
COL2A1 Fwd: 5′-CCAGATGACCTTCCTACGCC-3′
Rev: 5′-TTCAGGGCAGTGTACGTGAAC-3′
SOX9 Fwd: 5′-GTACCCGCACTTGCACAAC-3′
Rev: 5′-TCTCGCTCTCGTTCAGAAGTC-3′
PRG4 Fwd: 5′-CATGGAGTGCTGCCCTGATT-3′
Rev: 5′-TCTTACATTGGGCGTCGCAG-3′
COLX Fwd: 5′-CAC GTT TGG GTA GGC CTG TA-3′
Rev: 5′-TCT GTG AGC TCC ATG ATT GC-3′
ALPL Fwd: 5′-CCTCCTCGGAAGACACTCTG-3′
Rev: 5′-AGACTGCGCCTGGTAGTTGT-3′
IL1B Fwd: 5′-TAAGCCCACTCTACAGCTGG-3′
Rev: 5′-GAGAGGTGCTGATGTACCAG-3′
MMP13 Fwd: 5′-GACTGGTAATGGCATCAAGGGA-3′
Rev: 5′-CACCGGCAAAAGCCACTTTA-3′
TBP Fwd: 5′-TTG TAC CGCAGCTGCAAA AT-3′
Rev: 5′-TAT ATT CGG CGT TTC GGG CA-3′
GAPDH Fwd: 5′-CTGACTTCAACAGCGACACC-3′
Rev: 5′-CCCTGTTGCTGTAGCCAA AT-3′