TABLE 3.

CS/DS-degrading enzymes from animals.

Name	Substrate	Source	Degradation mechanism	Action pattern	EC number	References
HYAL1	HA or CS	human	hydrolysis	endo	EC 3.2.1.35 (GH56)	De Salegui and Pigman, 1967; Csoka et al., 1999
HYAL2	HA	human	hydrolysis	endo	EC 3.2.1.35 (GH56)	Rai et al., 2001; Csoka et al., 1999
HYAL3	HA	human	hydrolysis	endo	EC 3.2.1.35 (GH56)	Csoka et al., 1999
HYAL4	CS	human	hydrolysis	endo	EC 3.2.1.35 (GH56)	Csoka et al., 1999; Kaneiwa et al., 2010
PH-20	HA or CS	human	hydrolysis	endo	EC 3.2.1.35 (GH56)	Cherr et al., 2001
Hyaluronidase	HA	bovine testis	hydrolysis	endo	EC 3.2.1.35 (GH56)	Freeman et al., 1949
HYAL	HA	bee venom	hydrolysis	endo	EC 3.2.1.35 (GH56)	Gmach and Kreil, 1993
N-acetylgalactosamine-4-sulfatase (Arylsulfatase B, ARSB)	4-O-sulfate on GalNAc of CS and DS	human	sulfatase	exo (reducing end)	EC 3.1.6.12 (S1_2)	Baum et al., 1959; Wicker et al., 1991
N-acetylgalactosamine-6-sulfatase (GALNS)	6-O-sulfate on GalNAc of CS and keratan sulfate	human	sulfatase	exo (reducing end)	EC 3.1.6.4 (S1_5)	Lim and Horwitz, 1981; Bielicki and Hopwood, 1991; Tomatsu et al., 1991
Iduronate-2-sulfatase (IDS)	2-O-sulfate on IdoA of DS and Hep	human	sulfatase	exo (nonreducing end)	EC 3.1.6.13 (S1_7)	Lim et al., 1974; Shaklee et al., 1985; Wilson et al., 1990
Glucurono-2-sulfatase (Arylsulfatase K, ARSK)	2-O-sulfate on GlcA of HS	human	sulfatase	exo (nonreducing end)	EC 3.1.6.18 (S1_7)	Dhamale et al., 2017

GH: Glycoside hydrolase family, S: Sulfatase famlily.