FIGURE 7.

ROS regulated sevoflurane-induced Parthanatos in neuronal cells. (A) MTT analysis showed that reduction of cell viabilities induced by 4 and 8% sevoflurane for 12 h in SH-SY5Y cells, HT22 cells, and hippocampal neurons were markedly reversed by pretreatment of NAC. (B) LDH release assay showed that pretreatment of NAC markedly rescued cell death caused by 4 and 8% sevoflurane exposure for 12 h in SH-SY5Y cells, HT22 cells, and hippocampal neurons. (C–F) Western blotting and quantitative analysis showed that 4 and 8% sevoflurane for 12 h significantly upregulated the levels of cytoplasmic PAR polymer, PARP-1, and AIF both in cytoplasm and nucleus in SH-SY5Y cells, HT22 cells, and hippocampal neurons, which were markedly alleviated by pretreatment of NAC. Compared with control group, ^∗∗p < 0.01; Compared with sevoflurane group, significant differences were shown in neuronal cells treated with NAC prior to sevoflurane exposure (p < 0.01). Data are represented as mean ± SD from five independent experiments.