TABLE 5.

Recommendations for variable steps to maximize homology-directed repair (HDR) success in zebrafish.

inject into the 1 or 2-cell cytoplasm endonuclease mRNA ssDNA repair template symmetrical or asymmetrical 18-400 nucleotides for either arm interfering drugs may have a positive effect incubate injected embryos in drugs combination drugs recommended

See text for discussion. We compared all of the protocols and identified all of the steps where there was variability and analyzed those variables to determine what gave the best HDR rate.