FIGURE 1.

Genome organization of AfuPmV-1M. (A) dsRNA was extracted and electrophoresed on a 5% (w/v) polyacrylamide gel. (B) Schematic drawing of the genomic organization of AfuPmV-1M. “1”–“5” on the left indicates contigs. The horizontal lines and boxes indicate full dsRNA lengths and ORFs, respectively. The ORF sense orientations are from left to right for all contigs. Numbers on the lines and boxes indicate nucleotide numbers. Numbers under the boxes indicate amino acid (aa) lengths. (C) Left: Agarose gel electrophoresis of dsRNAs purified from the A. fumigatus strain infected with AfuPmV-1M (lane 1). Lane M, molecular weight marker. Right: Northern blot detection of dsRNA segments using digoxigenin-labeled DNA probes against dsRNA segments 1–5. dsRNA samples were electrophoresed on five different lanes of a single agarose gel and blotted. The five blotted lanes were separated and hybridized with the probes for each dsRNA. Lanes 1–5 of panel C were then reconstituted from the hybridized strips.