FIGURE 5.

Comparisons of virulence in mice of virus-free (Free) and virus-infected (Virus) strains. (A) Survival rates of mice infected with the AfuPmV-1M virus-infected and virus-free strains. ^∗p < 0.05 by the Kaplan–Meier log rank test. (B) CT scans of mouse chests at 14 days post-infection. (a–c) Virus-free fungal strain, (d–f) AfuPmV-1M virus-infected fungal strain. Shadows can be seen in the lungs of virus-free A. fumigatus-infected mice (arrowheads in a–c). (C) Fungal growth from the lungs of infected mice. Fungal burdens were estimated as CFUs per gram of lung. Data are presented as means ± SD of three independent experiments. ^∗p < 0.05 by two-tailed Student’s t-test. (D) Lung histology of mice at 3 days post-infection (Gomori’s methenamine silver-staining-Grocott’s variation). (a,b) virus-free strains; (c,d) virus-infected strains.