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. 2020 Dec 11;11:607795. doi: 10.3389/fmicb.2020.607795

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Copyright © 2020 Takahashi-Nakaguchi, Shishido, Yahara, Urayama, Ninomiya, Chiba, Sakai, Hagiwara, Chibana, Moriyama and Gonoi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological comparisons of AfuPmV-1M ORF expressing and non-expressing KU strains. (A) Colonies formed by native KU strain (KU), empty-vector expressing (−), and each AfuPmV-1M ORF-expressing KU strain (1–5). (B) Radial growth of colonies by ORF expressing (1–5) and non-expressing (−) KU strains. (C) Number of conidia formed by the strains at 3 days post- inoculation. (D) Percentages of swelling conidia at 6 h after the start of incubation. (E) Dry weight of mycelia. Mycelial growth was quantified by measuring their dry weight at 24 h after starting the incubation of conidia. Data are presented as means ± SD of three independent experiments. ^∗p < 0.05, by one-way ANOVA, Dunnett’s test.