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The phenotype-related proteins were screened by Western blot. Dkk3 overexpression and treatment decreased the expression of Bcl-2, cdc25B, cdc25c, N-cadherin, Slug, and Twist and increased the expression of Bax and E-cadherin in HCT-15 and HCT-116 cells. The anti-Dkk3 antibody suppressed the effects of Dkk3 overexpression on the expression of the phenotype-related proteins.
