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. 2020 Nov 30;10:600322. doi: 10.3389/fonc.2020.600322

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Copyright © 2020 Zhao, Hao, Zhao, Jiang and Zheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of Dkk3 on tumor growth and carcinogenesis of colorectal cancer. PCR primers were designed (A) and subjected to PCR of tail and stomach DNA (B) with the same color for the corresponding primers and products. Expression of Dkk3, N-cadherin, and E-cadherin was confirmed by real-time PCR (C) and Western blot (D). Immunohistochemistry was employed to observe the maker proteins’ expression in the intestinal adenoma of target villin-cre/Dkk3 knockout mice induced by sodium desoxycholate (E). *p < 0.05, compared with the transfectant or knockout mice. WT, wild-type mice; VD, villin-cre +; Dkk3 -/-.