Fig 3. Membrane Trafficking Proteome is Acquired After Maturation.

A: Workflow of the tandem mass tagging (TMT) based quantitative mass-spectrometry experiment. B: Maturity markers, Nav1.2 and pNfH are detected with higher abundances in mature SCG neurons. C: Gene Set Enrichment Analysis of the whole proteome (blue) reveals that immature SCG neurons are enriched (FDR 2.21×10⁻¹²) in transcription factors (red), and mature neurons are enriched (FDR 1.38×10⁻⁷) in membrane-trafficking associated proteins (yellow). D: Host proteins altered by infection. The heatmap graphs the log2 fold-change of host protein abundance values. Values are normalized to mock signal of the same age. All identified host proteins, that were found to be significantly differential (adjusted P-value ≤0.05) in a background-based ANOVA analysis in at least one comparison, were clustered with k means = 7. Cluster numbers correspond to the following enrichment GO-terms: 1-RNA splicing, 2-Metabolism and axon guidance, 3-Myelin sheath, 4-No enrichments, 5-RNA splicing. E: Viral proteins are more abundant in immature SCG neurons. The heatmap represents TMT reporter ion log2 fold-change values for PRV proteins. PRV proteins are temporally organized as IE (immediate early), E (early) and L (late) expressing.