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. 2021 Jan 9;226:122092. doi: 10.1016/j.talanta.2021.122092

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Fig. 1 — Single-nucleotide detection of KRAS using DCL in solution and bifunctional Strep-FAM-NPs (13). A) Work-flow scheme: 1) DCL reagents are added and DCL product is formed; 2) Strep-FAM-NPs (13) are added. These Strep-FAM-NPs (13) capture the DCL product. 3) Washing steps are performed; 4) Readout by FACS analysis. B) Schematic representation of the hybridisation step (I), followed by the dynamic incorporation of the biotin-labelled SMART-Nucleobase (II) and the reductive amination using NaBH₃CN (III). Finally, the DCL product is captured by the Strep-FAM-NPs (13) (IV).