Table 1.

Sequences of ssDNA, PNA, and DGL probes. KRAS codon 12 is shown in bold. The nucleotide under interrogation (nucleotide located in from of the PNA abasic position) is underlined in ssDNA sequences. The abasic position in the DGL probe is shown as *_*. Wt = wild-type sequence. G12C = presence of a mismatch (G > T) corresponding to the KRAS G12C point mutation. X = amino-PEG-linker; HAc = acetyl group; glu = nucleobase containing a propanoic acid side chain at the gamma position. Chemical structures are available in Supporting Information 1.

Target nucleic acid sequences.	Sequence (5′–3′)
ssDNA-KRAS-wt-Cy5	Cy5—TTGGAGCTGGTGGCGTAG
ssDNA-KRAS-G12C-Cy5	Cy5—TTGGAGCTTGTGGCGTAG
ssDNA-KRAS-wt-Biotin	Biotin—TTGGAGCTGGTGGCGTAG
PNA probes.	Sequence (N–C)
PNA-KRAS-wt-Biotin	Biotin—xxCTACGCCACCAGCTCCAA
PNA-KRAS-wt-Cy5	Cy5—xxCTACGCCACCAGCTCCAA
DGL probes.	Sequence (N–C)
DGL-K12RC	HAc –Cys-xxCTACGCCAgluC*_*AGgluCTCCAA