Table 2.
Methods |
RT-LAMP assay (I) |
RT-LAMP assay (II) |
Total | ||||
---|---|---|---|---|---|---|---|
Positive | Negative | Inconclusivea | Positive | Negative | |||
Real-time PCR assay | Positive | 173 (88.3%) | 23 (11.7%) | 4 | 191 (95.5%) | 9 (4.5%) | 200 |
Negative | 2 (2.9%) | 68 (97.1%) | – | 0 | 70 (100%) | 70b |
RT-LAMP, reverse transcription loop-mediated isothermal amplification assay. (I) the first interpretation; (II) the second interpretation.
Inconclusive refers to a color in a reaction tube indicating a weakly to moderately positive result, but a banding pattern appearing on the gel not specific for HCV amplification, or vice versa. After RT-LAMP retesting, three samples became positive, while one became negative.
These samples included 20 HIV-infected patient samples, 20 HBV-infected patient samples, and 30 healthy donor samples.